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Mouse Monoclonal IgG1 Anti-AGE-3 Glycolaldehyde modified [9D8]
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AGE3-010 Catalog number

10 µg Size

904 € Price

More details

Availability

Please contact us to check the availability

Immunogen

Glycolaldehyde-modified BSA

Specificity

AGE-3 (Glycolaldehyde-modified proteins); in Western Blot no cross-reaction with Glucose-modified AGEs (AGE-1), AGE-2 (Glycerolaldehyde-modified), AGE-4 (Methylglyoxal-modified), AGE-5 (Glyoxal-modified), AGE-6 (3-DG-modified), CEL-BSA, CML-BSA and BSA.

Species reactivity

not relevant

Product presentation

Protein G affinity purified monoclonal antibody in PBS with 2% Block Ace as stabilizer and 0.1% Proclin as preservative

Product category

Primary Antibody

Antibody's raised in

Mouse

Antibody's isotype

IgG1

Antibody's clone

9D8

Product is supplied as

liquid concentrate

Recommended concentration for use

1 µg/ml (WB) and 0,1 µg/ml (ELISA)

Protein concentration

0.25 mg/ml

Positive control

human lens, arteriosclerotic plaques

Tested applications

ELISA, WB

Recommended secondary reagent

As secondary system in ELISA and Western Blot we recommend biotinylated anti-mouse IgG antibody (Art. No. ZU102) in combination with streptavidin-HRPO conjugate (Art. No. ZU054) or streptavidin-alkaline phosphatase (Art. No. ZU051).

Storage method

Store at -20°C

UniProt number

Go to UniProt/SwissProt

Litterature

1. Yamagishi S., Amano S., Inagaki Y., Okamoto T., Koga K., Sasaki N., Yamamoto H., Takeuchi M., and Makita Z. (2002) Advanced Glycation End Products-Induced Apoptosis and Overexpression of Vascular Endothelial Growth Factor in Bovine Retinal Pericytes Biochemical and Biophysical Research Communications ; 290, 973-978. 2. Kume S., Kato S., Yamagishi S., Inagaki Y., Ueda S., Arima N., Okawa T., Kojiro M., Nagata K. (2005) Advanced Glycation End-Products Attenuate Human Mesenchymal Stem Cells and Prevent Cognate Differentiation Into Adipose Tissue, Cartilage, and Bone. J Bone Miner Res. 2005 Sep;20(9):1647-58. 3. Yamamoto Y., Yonekura H., Watanabe T., Sakurai S., Li H., Harashima A., Myint K.M., Osawa M., Takeuchi A., Takeuchi M., Yamamoto H. (2007) Short-chain aldehyde-derived ligands for RAGE and their actions on endothelial cells. Diabetes Res Clin Pract. 2007 Sep;77 Suppl 1:S30-40.

Tips

*Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus