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Mouse Monoclonal IgG1 Anti-Collagen Type VII, human [LH7.2]
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COL007 Catalog number

1 ml Size

413 € Price

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Availability

Please contact us to check the availability

Immunogen

Extract of human neonatal foreskin

Specificity

Collagen type VII, carboxyterminal

Species reactivity

Human, Marmoset, Monkey, Cow, Sheep, Goat, and Guinea pig

Product presentation

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.

Product category

Primary Antibody

Antibody's raised in

Mouse

Antibody's isotype

IgG1

Antibody's clone

LH7.2

Product is supplied as

liquid concentrate

Recommended concentration for use

1:10 - 1:20 IHC(C)

Protein concentration

50 µg/ml

Positive control

Normal skin

Pretreatment

Fixation with ice cold Acetone only (Methanol is not suitable!) No method known for paraffin sections!

Tested applications

IHC(C), IFA

Recommended secondary reagent

Anti-mouse IgG-conjugates, e.g. Anti-mouse IgG:FITC (Art. No. FI-2000) or anti-mouse IgG:DyLight488 (Art. No. DI-2488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7402).

Storage method

Store at 2-8°C

UniProt number

QGo to UniProt/SwissProt2388 (CO7A1_HUMAN)

Litterature

1. Hegerty A.M.H., et al. (1986) Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH7.2 monoclonal antibody: use in diagnosis. Br. J. Dermatol. 115; 125-131. 2. Leigh IM, Eady RA, Heagerty AH, Purkis PE, Whitehead PA, Burgeson RE (1988) Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa. J Invest Dermatol. 90(5): 3. Kirkham N., et al. (1989) Type VII collagen antibody, LH7.2 identifies basement membrane charakteristics of thin malignanat melanomas. J. Pathol. 157; 243-247.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Gene

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus