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Mouse Monoclonal IgG1 Anti-Cytokeratin 5 [RCK103]
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CK515 Catalog number

5 ml Size

250 € Price

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Availability

Please contact us to check the availability

Immunogen

Isolated cytokeratins from human epithelial cells

Specificity

Cytokeratin 5 and neurofilaments

Species reactivity

Human

Product presentation

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002 if required.

Product category

Primary Antibody

Antibody's raised in

Mouse

Antibody's isotype

IgG1

Antibody's clone

RCK103

Product is supplied as

ready to use

Recommended concentration for use

neat

Protein concentration

N/A

Positive control

Skin, Cervix

Pretreatment

Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C or G (Art. No. DE000 and DE007)

Tested applications

IHC(C, P)

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Litterature

1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Smedts F., Ramaekers F., Robben H., et al. (1990) Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J. Pathol. 136,3; 657-668. 3. Eyken P van, et al. (1990) A cytokeratin-immunohistochemical study of hepatoblastoma. Human Pathology 21; 302-308. 4. Broekaert D. Leigh I.M., Laue E.B., Van Muijen G.N.P., Ramackers F.C.S., De Bersaques J. and P. Couke (1993) An immunohistochemical and histochemical study of cytokeratin, involucrin and tranglutaminase in seborrhoeic keratosis. Arch dermatol. Res. 285; 482-490. 5. Verhagen A.P.M. Aalders, T.W., Ramaekers F.C.S., Debruyne F.M.J., and Schalken J.A. (1988) Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration. The Prostate 13; 25-38.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus