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Mouse Monoclonal IgG1 Anti-Desmin [D33]
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DE500 Catalog number

5 ml Size

192 € Price

More details

Availability

Please contact us to check the availability

Immunogen

Human purified Desmin from muscle cells

Specificity

Desmin, 53 kDa intermediary filament protein

Species reactivity

Human, chicken, hamster, rat

Product presentation

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.

Product category

Primary Antibody

Antibody's raised in

Mouse

Antibody's isotype

IgG1

Antibody's clone

D33

Product is supplied as

ready to use

Recommended concentration for use

neat

Protein concentration

N/A

Positive control

Appendix

Pretreatment

Pre-treatment with proteases is not recommended, unmasking fluid G (Art. No. DE007) or C (Art. No. DE000) may be helpful after long time formaldehyde fixation.

Tested applications

IHC(C, P)

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage method

Store at 2-8°C

UniProt number

P17661

Litterature

1. Van Muijen G.N.P., Ruiter D.J., and Warnaar S.O. (1987) Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab. Invest. 57; 359-369. 2. Pitt M.A., Roberts I.S.D., Agbamu D.A., and Eyden B.P. (1993) The nature of atypical multinucleated stromal cells: a study of 37 cases from different sites. Histopathol. 23; 137-145.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Gene

Desmins or DES proteins detect by anti-desmin antibodies and DES ELISA kits. DSM1 and DSM2 play an important role in Desminopathy. Desmin regulated Myofibrilate Myopathy is a protein filament mutation of muscle proteins.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus