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Mouse Monoclonal IgG1 Anti-HBsAg, Hepatitis B Surface Antigen [P3G9A8]
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HB500 Catalog number

5 ml Size

146 € Price

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Availability

Please contact us to check the availability

Immunogen

Purified Hepatitis B surface antigen

Specificity

HBsAg subtypes ad and ay

Species reactivity

Hepatitis B Virus

Product presentation

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.

Product category

Primary Antibody

Antibody's raised in

Mouse

Antibody's isotype

IgG1

Antibody's clone

P3G9A8

Product is supplied as

ready to use

Recommended concentration for use

neat

Protein concentration

N/A

Positive control

HBV-infected liver

Pretreatment

No protease pre-treatment required! Blocking of endogenous peroxidase with 1% peroxide in methanol!

Tested applications

IHC(P)

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Litterature

1. Beasley R.P., et al. (1981) Hepatocellular carcinoma and hepatitis B virus: a prospective study of 22,707 men in Taiwan. Lancet ii; 1129 2. Hoofnagel J.H., and Alter H.J. (1984) Chronic viral hepatitis. In: Vyas G.N. et al. Eds. Viral hepatitis and liver disease. Grune & Stratton, N.Y. pp 97 ff. 3. Swensen P.D. et al. (1988). Antigenic analysis of HBsAg with monoclonal antibodies specific for S protein and pre-S2. Viral hepatitis and liver disease. Alan R. Liess, Inc. pp. 627ff.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Virus

hepatitis

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native dependent on the production method.Hepatitis a, b, c, d, e recombinant surface viral antigens to genotype 1, 2, 3, 4, 5 are used for antibody production to make diagnostic poly or monoclonal antibodies. Hepatitis is a disease of the liver characterized by the presence of inflammatory cells in the tissue of the organ. Hepatitis may occur without symptoms, but can lead to jaundice (a yellow discoloration of the skin, mucous membranes, and conjunctiva of the eyes), poor appetite, and fatigue. Depending on the cause, hepatitis can manifest either as an acute or as a chronic disease.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus