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Aggrecanase Assay ELISA (Activity Assay) Kit
from  biotez
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30 510 111 Catalog number

1 Microplate/ 96 determinations Size

569 € Price

More details

ELISA category

ELISA (Activity Assay) Kit

ELISA detection limits

0.024 - 1.5 nM ADAMTS4

ELISA sensitivity

12 pM ADAMTS4

ELISA's description

The assay consists of two moduls, the Aggrecanase Modul and the ELISA Module. A recombinant fragment of human aggrecan inter-globular domain (aggrecan-IGD) is first digested with aggrecanase. Proteolytic cleavage of the substrate releases an aggrecan peptide with the N-terminal sequence ARGSVIL (ARGSVIL-peptide). Subsequently, ARGSVIL-neoepitope containing cleavage products are detected and quantified by ELISA using two monoclonal anti-peptide antibodies .

Research area interests

Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology

Package form

96 well microplate

Antigen

Aggrecanase

Reacts with

Aggrecanase

Tested applications

The assay measures activity of aggrecanases. It is used to screen and characterize aggrecanase inhibitors.

Specificity

Aggrecanase

Crossreactivity

N/A

Preservative materials

If applicable mentioned in the product data sheet

Storage recommendations

6 month

Shipping and storage conditions

dry Ice and ice pack

Long term storage

-20 °C and 4 °C

Assay principle

Proteolytic Reaction and Sandwich-ELISA

Included in the standard package

microplate, buffers, antibody conjugate,standards

Not included in the standard package

Spectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water

Samples to be analyzed

cell culture

PubMed citations

See the datasheet

Technical datasheet

Contact us to receive the datasheet

Representative figures

Warnings

For Research Use only.

Test

biotez supplies other types of Assays as 1.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays