1.25 - 80 ng/ml HtrA4
The quantitative assay is based on a sandwich format. A highly specific monoclonal antibody for the HtrA4 is immobilized on the plate. HtrA4 is bound to the immobilized antibody, other components of the sample are removed by aspiration and washing. The analyte is detected in two steps using a secondary biotin-labeled antibody and a highly polymerized streptavidin-peroxidase conjugate. Any reagent excess is removed by aspiration and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition od TMB Substrate. The reaction is stopped by adding the Stop Solution, and the resultant color is read in a microtiter plate reader at 450 nm. The concentration of HtrA4 in a sample is determined by interpolation from the standard curve.
Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
96 well microplate
The system provides a highly sensitive and specific quantitative determination of HtrA4 (80 ng/ml - 1.25 ng/ml) in serum, tissue e.g. placenta and cell culture supernatants.
If applicable mentioned in the product data sheet
microplate, buffers, antibody conjugate,standards
Spectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
biological fluids, cell culture
See the datasheet
Contact us to receive the datasheet
For Research Use only.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED