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HtrA4 ELISA Kit
from  biotez
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30 516 401 Catalog number

1 Microplate/96 determinations Size

485 € Price

More details

ELISA category

ELISA Kit

ELISA detection limits

1.25 - 80 ng/ml HtrA4

ELISA sensitivity

1 ng/ml

ELISA's description

The quantitative assay is based on a sandwich format. A highly specific monoclonal antibody for the HtrA4 is immobilized on the plate. HtrA4 is bound to the immobilized antibody, other components of the sample are removed by aspiration and washing. The analyte is detected in two steps using a secondary biotin-labeled antibody and a highly polymerized streptavidin-peroxidase conjugate. Any reagent excess is removed by aspiration and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition od TMB Substrate. The reaction is stopped by adding the Stop Solution, and the resultant color is read in a microtiter plate reader at 450 nm. The concentration of HtrA4 in a sample is determined by interpolation from the standard curve.

Research area interests

Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology

Package form

96 well microplate

Antigen

HtrA4

Reacts with

HtrA4

Tested applications

The system provides a highly sensitive and specific quantitative determination of HtrA4 (80 ng/ml - 1.25 ng/ml) in serum, tissue e.g. placenta and cell culture supernatants.

Specificity

HtrA4

Crossreactivity

N/A

Preservative materials

If applicable mentioned in the product data sheet

Storage recommendations

6 month

Shipping and storage conditions

ice pack

Long term storage

4 °C

Assay principle

Sandwich-ELISA

Included in the standard package

microplate, buffers, antibody conjugate,standards

Not included in the standard package

Spectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water

Samples to be analyzed

biological fluids, cell culture

PubMed citations

See the datasheet

Technical datasheet

Contact us to receive the datasheet

Representative figures

Warnings

For Research Use only.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED