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Biotin ELISA Kit
from  abebio
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AE29927BI-96 Catalog number

1x plate of 96 wells Size

671 € Price

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Description

Biotin ELISA Kit is manufactured by highest quality antibodies and plates to provide you with excellent and reproducible results in your work. The specifically designed buffers will ensure optimal conditions in each step from diluting the samples, through the incubation to washing.

Storage and shipping

Store and ship all of of the comptents of the EIA assay for Biotin on blue ice/ice packs at +4 degrees Celcius. Afoid freezing and especially freeze-thaw cycles as such cycles may denaturate the peptide chains in the antibodies, standards and enzymes, thus reducing the activity and sensitivity of the kit.

Advise tips

Small volumes of the liquid components of the Biotin ELISA Kit may get caught on the vials' walls and seals. Prior to use, briefly centrifuge the vials to ensure that all of the vial's content is on the bottom of the vial.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Conjugation

Biotinylated

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED